transformed human liver epithelial-2 cells  (iCell Bioscience Inc)

Journal: Cell Division

Article Title: Overexpression of ZFP69B promotes hepatocellular carcinoma growth by upregulating the expression of TLX1 and TRAPPC9

doi: 10.1186/s13008-024-00131-z

Figure Lengend Snippet: Overexpression of TLX1 is identified in HCC tissues and cell lines. ( A ) Transcriptome differences between HCC and paracancerous tissues in the GSE87410 dataset. ( B ) Transcriptome differences between HCC and adjacent non-tumor in the GSE105130 dataset. ( C ) Intersection of the above two datasets with human transcription factors and transcription cofactors in Jvenn. ( D ) KEGG pathway enrichment analysis of 225 intersecting genes. ( E ) Heatmaps of 11 intersecting genes enriched in the Transcriptional misregulation in cancer pathway in the GSE87410 and GSE105130 datasets. ( F ) mRNA expression of TLX1 in HCC tissues and their adjacent tissues was analyzed using RT-qPCR ( n = 31). ( G ) positive staining of TLX1 in HCC tissues and their adjacent tissues was analyzed using immunohistochemistry ( n = 31). ( H ) TLX1 mRNA expression in THLE-2, Huh-7, and MHCC97H cells was analyzed using RT-qPCR. Data represent the mean ± SEM of at least three independent experiments. **** p < 0.0001. Differences were tested using a paired t-test (F, G) and the one-way ANOVA ( H )

Article Snippet: Transformed human liver epithelial-2 (THLE-2) cells (CL-0833) were purchased from Procell (Wuhan, Hubei, China) and cultured in a specialized medium (CM-0833) provided by the manufacturer.

Techniques: Over Expression, Expressing, Quantitative RT-PCR, Staining, Immunohistochemistry

Journal: Cell Division

Article Title: Overexpression of ZFP69B promotes hepatocellular carcinoma growth by upregulating the expression of TLX1 and TRAPPC9

doi: 10.1186/s13008-024-00131-z

Figure Lengend Snippet: TLX1 activates TRAPPC9 transcription in HCC cells. ( A ) The intersection of TLX1 downstream targets in hTFtarget and differentially expressed genes in the GSE87410 and GSE105130 datasets. ( B ) The heatmaps of 8 intersecting targets in the three datasets. ( C ) The binding relation between TLX1 and the TRAPPC9 promoter was predicted in the UCSC database. ( D ) Expression of TRAPPC9 mRNA in HCC tissues and their adjacent tissues was analyzed using RT-qPCR ( n = 31). (E) positive staining of TRAPPC9 in HCC tissues and their adjacent tissues was analyzed using immunohistochemistry ( n = 31). ( F ) Expression of TRAPPC9 mRNA in THLE-2, Huh-7, and MHCC97H was analyzed using RT-qPCR. ( G ) Enrichment of the TRAPPC9 promoter in HCC cells with anti-TLX1 antibody or anti-IgG control was analyzed using ChIP. ( H ) Jaspar database analysis of binding sites between TLX1 and the TRAPPC9 promoter ( I ) The binding between TLX1 and TRAPPC9 was assessed using EMSA. ( J ) The binding relation between TRAPPC9 and TLX1 was examined using a luciferase reporter assay. ( K ) Expression of TRAPPC9 mRNA in Huh-7 and MHCC97H infected with oe-TLX1 was analyzed using RT-qPCR. ( L ) TRAPPC9 mRNA expression after knockdown of TLX1 or TRAPPC9 in Huh-7 and MHCC97H cells was analyzed using RT-qPCR Data represent the mean ± SEM of at least three independent experiments. ** p < 0.01, *** p < 0.001, **** p < 0.0001. Differences were tested using t-tests ( D , E , G , J , K ) and the one-way ANOVA ( F , L )

Article Snippet: Transformed human liver epithelial-2 (THLE-2) cells (CL-0833) were purchased from Procell (Wuhan, Hubei, China) and cultured in a specialized medium (CM-0833) provided by the manufacturer.

Techniques: Binding Assay, Expressing, Quantitative RT-PCR, Staining, Immunohistochemistry, Control, Luciferase, Reporter Assay, Infection, Knockdown

Journal: Cell Division

Article Title: Overexpression of ZFP69B promotes hepatocellular carcinoma growth by upregulating the expression of TLX1 and TRAPPC9

doi: 10.1186/s13008-024-00131-z

Figure Lengend Snippet: ZFP69B transcriptionally activates TLX1 expression in HCC cells. ( A ) The intersection of TFs with binding sites in the TLX1 promoter and enhancer regions downloaded on GeneCards and differentially expressed genes in the GSE87410 and GSE105130 datasets. ( B ) The heatmaps of intersecting TF expression in the GSE87410 and GSE105130 datasets. ( C ) The ZFP69B binding peaks on the TLX1 promoter were downloaded from the UCSC database. ( D ) Expression of ZFP69B mRNA in HCC tissues and their adjacent tissues was examined using RT-qPCR ( n = 31). ( E ) positive staining of ZFP69B in HCC tissues and their adjacent tissues was analyzed using immunohistochemistry ( n = 31). ( F ) ZFP69B mRNA in THLE-2, Huh-7, and MHCC97H cells was examined using RT-qPCR. ( G ) Enrichment of the TLX1 promoter in HCC cells with anti-ZFP69B antibody or anti-IgG control was analyzed using ChIP. ( H ) The binding relation between ZFP69B and TLX1 was examined using a luciferase reporter assay. ( I ) TLX1 mRNA in Huh-7 and MHCC97H cells infected with sh-ZFP69B was examined using RT-qPCR. Data represent the mean ± SEM of at least three independent experiments. ** p < 0.01, *** p < 0.001, **** p < 0.0001. Differences were tested using a t-test (D, E, G, H, I) and the one-way ANOVA (F)

Article Snippet: Transformed human liver epithelial-2 (THLE-2) cells (CL-0833) were purchased from Procell (Wuhan, Hubei, China) and cultured in a specialized medium (CM-0833) provided by the manufacturer.

Techniques: Expressing, Binding Assay, Quantitative RT-PCR, Staining, Immunohistochemistry, Control, Luciferase, Reporter Assay, Infection